Human SPARC ELISA Kit
Cat no : KE00903
Synonyms
Basement membrane protein 40, Basement-membrane protein 40, BM 40, BM-40, ON
Validation Data Gallery
Product Information
KE00903 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human SPARC ELISA kit is to be used to detect and quantify protein levels of endogenous human SPARC. The assay recognizes human SPARC. An antibody specific for human SPARC has been pre-coated onto the microwells. The human SPARC protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human SPARC is added to detect the captured human SPARC protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SPARC ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Human milk |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6678 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 91% | 87%-97% |
| Cell culture supernatant | 92% | 90%-95% |
| Human milk | 76% | 75%-79% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,462.81 | 59.40 | 4.06 |
| 2 | 8 | 354.62 | 11.06 | 3.12 |
| 3 | 8 | 83.84 | 3.86 | 4.60 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,542.67 | 130.69 | 8.47 |
| 2 | 16 | 368.44 | 22.78 | 6.18 |
| 3 | 16 | 85.75 | 5.98 | 6.97 |
Background Information
SPARC, also known as ON (Osteonectin) or BM-40 (Basement-membrane protein 40), is an extracellular glycoprotein. SPARC belongs to a group of matricellular proteins defined as secreted components that do not contribute directly to the formation of structural elements but serve to modulate cell-matrix interactions and cellular functions. SPARC is expressed at high levels in bone tissue, is distributed widely in many other tissues and cell types, and is associated generally with tissues undergoing morphogenesis, remodeling and wound repair. It elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix. Altered expression of SPARC has been reported in a variety of cancers, which include breast, ovarian, colorectal, and pancreatic cancer as well as melanoma and glioblastomas.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Basement membrane protein 40, Basement-membrane protein 40, BM 40, BM-40, ON |