Human SRP54 ELISA Kit
Cat no : KE00462
Synonyms
SRP54, EC:3.6.5.4, SCN8, Signal recognition particle subunit SRP54
Validation Data Gallery
Product Information
KE00462 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human SRP54 ELISA kit is to be used to detect and quantify protein levels of endogenous human SRP54. The assay recognizes human SRP54. An antibody specific for human SRP54 has been pre-coated onto the microwells. The human SRP54 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human SRP54 is added to detect the captured human SRP54 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SRP54 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.15 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6729 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 108% | 98%-115% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 10.74 | 0.67 | 6.26 |
| 2 | 8 | 3.06 | 0.10 | 3.19 |
| 3 | 8 | 0.81 | 0.04 | 5.42 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 11.49 | 1.20 | 10.46 |
| 2 | 16 | 2.81 | 0.27 | 9.61 |
| 3 | 16 | 0.84 | 0.06 | 6.90 |
Background Information
Signal Recognition Particle 54 kDa (SRP54) is a subunit of the signal recognition particle (SRP), a cytoplasmic ribonucleoprotein complex guiding the transportation of newly synthesized proteins from polyribosomes to endoplasmic reticulum. SRP54 plays a role in granulocyte prolif eration and differentiation, neutrophil migration capacity and pancre atic exocrine development. In addition, it has been shown that signal recognition particle 54 (SRP54) acts as a negative regulator of antiviral signal transduction induced by RLRs.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | SRP54, EC:3.6.5.4, SCN8, Signal recognition particle subunit SRP54 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.