Human Serotonin transporter ELISA Kit
Cat no : KE00854
Synonyms
SLC6A4, 5 HTT, 5 HTTLPR, 5HTT, hSERT
Validation Data Gallery
Product Information
KE00854 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Serotonin transporter ELISA kit is to be used to detect and quantify protein levels of endogenous human Serotonin transporter. The assay recognizes human Serotonin transporter. An antibody specific for human Serotonin transporter has been pre-coated onto the microwells. The human Serotonin transporter protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Serotonin transporter is added to detect the captured human Serotonin transporter protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Serotonin transporter ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.7 pg/mL |
| Range | 3.9-250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6532 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 90% | 84%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 128.9 | 3.7 | 2.8 |
| 2 | 8 | 34.8 | 0.7 | 1.9 |
| 3 | 8 | 18.6 | 0.4 | 1.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 127.4 | 3.7 | 2.9 |
| 2 | 16 | 34.5 | 0.9 | 2.5 |
| 3 | 16 | 18.5 | 0.4 | 2.2 |
Background Information
The serotonin transporter (SERT) is a crucial protein encoded by the SLC6A4 gene, responsible for regulating serotonin levels in the brain and gut. It plays a significant role in mood regulation, anxiety, and gastrointestinal functions. SERT is also a key target for antidepressants and other psychoactive drugs. Genetic variations of SERT are associated with mental disorders.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | SLC6A4, 5 HTT, 5 HTTLPR, 5HTT, hSERT |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.