Human Siglec-7/CD328 ELISA Kit
Cat no : KE00661
Synonyms
SIGLEC7, Siglec-7, AIRM 1, AIRM1, AIRM-1
Validation Data Gallery
Product Information
KE00661 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Siglec-7/CD328 ELISA kit is to be used to detect and quantify protein levels of endogenous human Siglec-7/CD328. The assay recognizes human Siglec-7/CD328. An antibody specific for human Siglec-7/CD328 has been pre-coated onto the microwells. The human Siglec-7/CD328 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Siglec-7/CD328 is added to detect the captured human Siglec-7/CD328 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Siglec-7/CD328 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 11.4 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 27036 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 94% | 86%-107% |
| Lysate | 102% | 95%-110% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,189.2 | 40.0 | 3.4 |
| 2 | 8 | 297.8 | 7.0 | 2.4 |
| 3 | 8 | 161.3 | 3.4 | 2.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,246.5 | 66.3 | 5.3 |
| 2 | 16 | 309.4 | 13.3 | 4.3 |
| 3 | 16 | 169.7 | 10.2 | 6.0 |
Background Information
Sialic acid-binding Ig-like lectin 7 (Siglec-7, also known as CD328) is an inhibitory receptor expressed on natural killer (NK) cells, monocytes, macrophages, dendritic cells, and mast cells. It plays a crucial role in regulating immune responses by binding to sialic acid-containing glycans on the surface of target cells, including cancer cells and pathogens. Moreover, Siglec-7 is emerging as a very important regulator of the immune response, which shows that increased expression of sialylated ligands on tumour cells inhibits NK cell activation through engagement of Siglec-7.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | SIGLEC7, Siglec-7, AIRM 1, AIRM1, AIRM-1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.