Human Siglec-8 ELISA Kit
Cat no : KE00539
Synonyms
SIGLEC8, Siglec-8, SAF 2, SAF2, Sialic acid-binding Ig-like lectin 8
Validation Data Gallery
Product Information
KE00539 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Siglec-8 ELISA kit is to be used to detect and quantify protein levels of endogenous human Siglec-8. The assay recognizes human Siglec-8. An antibody specific for human Siglec-8 has been pre-coated onto the microwells. The human Siglec-8 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Siglec-8 is added to detect the captured human Siglec-8 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Siglec-8 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 6.9 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 27181 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 94% | 70%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,854.7 | 219.7 | 7.7 |
| 2 | 8 | 643.6 | 32.5 | 5.0 |
| 3 | 8 | 179.5 | 11.9 | 6.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,949.6 | 289.8 | 9.8 |
| 2 | 16 | 694.8 | 62.7 | 9.0 |
| 3 | 16 | 198.1 | 21.8 | 11.0 |
Background Information
Sialic acid-binding Ig-like lectin 8 (Siglec-8) is a novel sialic acid-binding immunoglobulin-like lectin that is uniquely expressed by human eosinophils, mast cells, as well as basophils. It has homology with other sialoadhesin family members (CD33 and siglec-5) and belongs to a subgroup of the Ig superfamily. Siglec-8 also exists in a soluble form in human serum (sSiglec-8), and reports suggested that sSiglec-8 might be used as a biomarker for various eosinophil-associated diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | SIGLEC8, Siglec-8, SAF 2, SAF2, Sialic acid-binding Ig-like lectin 8 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.