Human TFF1 ELISA Kit
Cat no : KE00370
Synonyms
TFF1,Breast cancer estrogen-inducible protein,HP1.
Validation Data Gallery
Product Information
KE00370 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TFF1 ELISA kit is to be used to detect and quantify protein levels of endogenous human TFF1. The assay recognizes human TFF1. An antibody specific for human TFF1 has been pre-coated onto the microwells. The human TFF1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human TFF1 is added to detect the captured human TFF1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TFF1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine |
| Assay type | Sandwich |
| Sensitivity | 1.4 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7031 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| serum | 89% | 77%-99% |
| Urine | 99% | 92%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 965.3 | 54.8 | 5.7 |
| 2 | 20 | 204.4 | 7.5 | 3.7 |
| 3 | 20 | 46.8 | 3.9 | 8.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,012.1 | 58.0 | 5.7 |
| 2 | 24 | 243.5 | 13.0 | 5.3 |
| 3 | 24 | 57.6 | 2.5 | 4.4 |
Background Information
Trefoil factor 1 (TFF1) is also named as PNR-2, Polypeptide P1.A (hP1.A), Breast cancer estrogen-inducible protein (BCEI) and Protein pS2. TFF1 is one of three members of the trefoil factor family. While abundant TFF1 expression can be observed in the gastrointestinal epithelium, it is also expressed in other organs, such as the ileum, colon, and pancreas. The known function of TFF1 is to promote the regeneration of gastric mucosa induced by inflammatory injury by encouraging epithelial cell migration, and a similar regenerative effect is also found in the pancreas and liver. Importantly, recent studies have revealed that TFF1 also functions as a tumor suppressor to inhibit gastric, pancreatic, and hepatocellular carcinogenesis, presumably by inhibiting the Wnt/β-catenin pathway. In terms of the esophagus, TFF1 is not expressed in the squamous epithelium of the esophagus but is expressed in the submucosal gland and BE.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TFF1,Breast cancer estrogen-inducible protein,HP1. |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.