Human THOC1 ELISA Kit
Cat no : KE00863
Synonyms
Nuclear matrix protein p84, HPR1, p84N5, Tho 1, THO complex 1
Validation Data Gallery
Product Information
KE00863 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human THOC1 ELISA kit is to be used to detect and quantify protein levels of endogenous human THOC1. The assay recognizes human THOC1. An antibody specific for human THOC1 has been pre-coated onto the microwells. The human THOC1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human THOC1 is added to detect the captured human THOC1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human THOC1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.09 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9984 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 80% | 76%-86% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 8.61 | 0.32 | 3.77 |
| 2 | 8 | 2.21 | 0.13 | 6.01 |
| 3 | 8 | 1.19 | 0.10 | 8.70 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 9.28 | 0.80 | 8.66 |
| 2 | 16 | 2.24 | 0.12 | 5.25 |
| 3 | 16 | 1.18 | 0.10 | 8.81 |
Background Information
The THO Complex 1 (THOC1) is part of the THO ribonucleoprotein complex that is cotranscriptionally assembled on nascent RNA transcripts, facilitating its interaction with RNA processing and export factors. THOC1 expression is elevated in many human cancers, such as prostate, colorectal and lung cancers , and associated with poor prognosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Nuclear matrix protein p84, HPR1, p84N5, Tho 1, THO complex 1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.