Human THOP1 ELISA Kit
Cat no : KE00889
Synonyms
EC:3.4.24.15, EP24.15, MEPD_HUMAN, MP78, THOP 1
Validation Data Gallery
Product Information
KE00889 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human THOP1 ELISA kit is to be used to detect and quantify protein levels of endogenous human THOP1. The assay recognizes human THOP1. An antibody specific for human THOP1 has been pre-coated onto the microwells. The human THOP1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human THOP1 is added to detect the captured human THOP1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human THOP1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.07 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7064 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 76% | 72%-81% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 26.10 | 1.50 | 5.75 |
| 2 | 8 | 7.19 | 0.43 | 6.00 |
| 3 | 8 | 3.73 | 0.24 | 6.46 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 25.15 | 1.72 | 6.83 |
| 2 | 16 | 6.74 | 0.59 | 8.78 |
| 3 | 16 | 3.42 | 0.40 | 11.64 |
Background Information
Thimet oligopeptidase (THOP1), also known as EP24.15, MP24.15, and EC 3.4.24.15, belongs to the M3 family of zinc-dependent endopeptidases. It moves between the cytoplasm and nucleus of neurons and glial cells and is secreted into extracellular space via multiple mechanisms. Its function in major histocompatibility class I antigen presentation has been well studied, and more recently discovered roles in hyperlipidic diet-induced obesity, non-alcoholic liver steatosis, and insulin resistance are under investigation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:3.4.24.15, EP24.15, MEPD_HUMAN, MP78, THOP 1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.