Human TIGAR ELISA Kit
Cat no : KE00989
Synonyms
C12orf5, EC:3.1.3.46, Fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, TP53-induced glycolysis regulatory phosphatase
Validation Data Gallery
Product Information
KE00989 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TIGAR ELISA kit is to be used to detect and quantify protein levels of endogenous human TIGAR. The assay recognizes human TIGAR. An antibody specific for human TIGAR has been pre-coated onto the microwells. The human TIGAR protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human TIGAR is added to detect the captured human TIGAR protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TIGAR ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 57103 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 96% | 90%-105% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.23 | 0.14 | 2.71 |
| 2 | 8 | 1.35 | 0.03 | 2.22 |
| 3 | 8 | 0.73 | 0.02 | 3.04 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.14 | 0.18 | 3.56 |
| 2 | 16 | 1.36 | 0.03 | 2.17 |
| 3 | 16 | 0.73 | 0.03 | 3.44 |
Background Information
TP53-induced glycolysis and apoptosis regulator (TIGAR) is a downstream target gene of p53 and an important factor involved in glycolysis and apoptosis. It is indispensable in metabolism and is involved in metabolic syndrome, including hyperglycemia, insulin resistance, alcoholic fatty liver and tissue ischemia. TIGAR is highly expressed in many cancer cells to promote cell survival. TIGAR can aggravate myocardial ischemia injury and heart failure in animal and cell models.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C12orf5, EC:3.1.3.46, Fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, TP53-induced glycolysis regulatory phosphatase |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.