Human TIM3 ELISA Kit
Cat no : KE00550
Synonyms
TIM3, CD366, HAVCR2, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3
Validation Data Gallery
Product Information
KE00550 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TIM3 ELISA kit is to be used to detect and quantify protein levels of endogenous human TIM3. The assay recognizes human TIM3. An antibody specific for human TIM3 has been pre-coated onto the microwells. The human TIM3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human TIM3 is added to detect the captured human TIM3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TIM3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine |
| Assay type | Sandwich |
| Sensitivity | 1.8 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 84868 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 87% | 80%-94% |
| Cell culture supernatant | 101% | 97%-105% |
| Urine | 109% | 106-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,016.0 | 37.0 | 3.6 |
| 2 | 8 | 252.6 | 6.3 | 2.5 |
| 3 | 8 | 125.6 | 6.1 | 4.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 954.6 | 78.4 | 8.2 |
| 2 | 16 | 250.1 | 11.2 | 4.5 |
| 3 | 16 | 124.3 | 7.3 | 5.8 |
Background Information
TIM3 (T-cell immunoglobulin and mucin-domain containing molecule 3), also known as HAVCR2, is an immune checkpoint receptor expressed on various immune cells, including T cells, dendritic cells, and macrophages. It plays a crucial role in regulating immune responses by mediating inhibitory signals contributing to immune tolerance and exhaustion. TIM3 is highly expressed on tumor-infiltrating lymphocytes (TILs) and is associated with poor prognosis in several cancers, including colorectal cancer. In cancer, soluble TIM3 may contribute to immune evasion by binding to galectin-9 and other ligands, thereby inhibiting the function of immune cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TIM3, CD366, HAVCR2, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.