Human TIMM44 ELISA Kit
Cat no : KE00947
Synonyms
MIMT44, TIM44, TIMM 44
Validation Data Gallery
Product Information
KE00947 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TIMM44 ELISA kit is to be used to detect and quantify protein levels of endogenous human TIMM44. The assay recognizes human TIMM44. An antibody specific for human TIMM44 has been pre-coated onto the microwells. The human TIMM44 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human TIMM44 is added to detect the captured human TIMM44 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TIMM44 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.07 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10469 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 108% | 89%-127% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 9.24 | 0.52 | 5.66 |
| 2 | 8 | 2.50 | 0.08 | 3.24 |
| 3 | 8 | 1.39 | 0.05 | 3.31 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 9.43 | 0.52 | 5.51 |
| 2 | 16 | 2.45 | 0.08 | 3.38 |
| 3 | 16 | 1.37 | 0.05 | 3.99 |
Background Information
TIMM44 (translocase of inner mitochondrial membrane 44) locates in the inner mitochondrial membrane . In a ATP-dependent manner, TIMM44 anchors mitochondrial heat shock protein 70 to the translocase of the mitochondrial inner membrane 23 (TIMM23) complex . TIMM44 modulates mitochondrial pre-proteins' import to the mitochondrial matrix. This process requires appropriate inner membrane potential and ATP production through mtHsp70.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MIMT44, TIM44, TIMM 44 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.