Speedy™ Human/Cynomolgus Monkey TIMP1 One-Step ELISA Kit
Cat no : SE50010
Synonyms
TIMP-1, CLGI, Collagenase inhibitor, EPA, Erythroid-potentiating activity
Validation Data Gallery
Product Information
| Product name | Speedy™ Human/Cynomolgus Monkey TIMP1 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, Human plasma, Cynomolgus monkey serum, Cynomolgus monkey plasma, Cell culture supernatant, Saliva |
| Assay type | Sandwich |
| Sensitivity | 2.4 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human/Cynomolgus Monkey |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7076 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 105% | 98%-114% |
| Saliva | 103% | 98%-111% |
| Cell culture supernatant | 94% | 86%-98% |
| Cynomolgus monkey serum | 97% | 90%-104% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 443.5 | 10.6 | 2.4 |
| 2 | 8 | 115.2 | 1.9 | 1.7 |
| 3 | 8 | 35.4 | 0.4 | 1.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 447.7 | 14.7 | 3.3 |
| 2 | 16 | 116.2 | 2.3 | 2.0 |
| 3 | 16 | 36.2 | 1.6 | 4.3 |
Background Information
Metalloproteinase inhibitor 1 (TIMP1) is also named as Erythroid-potentiating activity (EPA), Fibroblast collagenase inhibitor (Collagenase inhibitor) and CLGI. TIMP1 is one of the tissue inhibitor members of the metalloproteinase family. TIMP1 regulates the balance of matrix remodeling during the degradation of extracellular matrix by inhibiting the proteolytic activity of matrix metalloproteinases (MMPs). TIMP1 promotes the occurrence and development of GC, which is consistent with previous reports about TIMP1 as an oncogene in other cancers, including breast cancer, colon cancer, glioblastoma, and non-small cell lung cancer. TIMP1 is reportedly as a tumor-secreted protein that could be detected in the serum. In a diabetic environment, TIMP1 initially binds to TFRC, leading to increased TFRC expression, thereby heightening osteoblast sensitivity to ferroptosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TIMP-1, CLGI, Collagenase inhibitor, EPA, Erythroid-potentiating activity |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.