Human TIMP4 ELISA Kit
Cat no : KE00198
Synonyms
Metalloproteinase inhibitor 4, TIMP 4, TIMP4, TIMP-4
Validation Data Gallery
Product Information
KE00198 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TIMP4 ELISA kit is to be used to detect and quantify protein levels of endogenous TIMP4. The assay recognizes human TIMP4. An antibody specific for TIMP4 has been pre-coated onto the microwells. The TIMP4 protein in samples is captured by the coated antibody after incubation.Following extensive washing, another antibody of biotinylated specific for TIMP4 is added to detect the captured TIMP4 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TIMP4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Human milk |
| Assay type | Sandwich |
| Sensitivity | 4.1 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7079 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 102% | 77%-116% |
| cell culture supernatants | 113% | 88%-129% |
| Human milk | 102% | 88%-127% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 480.5 | 24.5 | 5.1 |
| 2 | 20 | 893.5 | 58.5 | 6.5 |
| 3 | 20 | 3,844.1 | 208.5 | 5.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 515.1 | 51.2 | 9.9 |
| 2 | 24 | 1,058.1 | 67.8 | 6.4 |
| 3 | 24 | 4,065.2 | 271.9 | 6.7 |
Background Information
Endogenous inhibitors of matrix metalloproteinases (MMPs) called tissue inhibitors of matrix metalloproteinases (TIMPs) form a family of four members,TIMP1-4. Although possessing many similarities, TIMPs show several structural and biochemical differences, and their expression patterns differ. TIMP4 is characterized by being primarily restricted to cardiovascular structures. Experimental studies have suggested a role for TIMP4 in cardiovascular disorders exhibiting an inflammatory component. Overexpression of TIMP4 has been observed within the arterial wall in rats after a balloon injury. TIMP4 has been shown to suppress vascular smooth muscle cell migration and induce cell apoptosis and to inhibit capillary endothelial cell migration.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Metalloproteinase inhibitor 4, TIMP 4, TIMP4, TIMP-4 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
J Inflamm Res TIMP4 as a Potential Complementary Biomarker and Therapeutic Target in Membranous Nephropathy: A Multi-Omics Investigation with Clinical Validation. |