Human TK1 ELISA Kit
Cat no : KE00285
Synonyms
thymidine kinase 1, soluble, Thymidine kinase, cytosolic, TK1, TK2
Validation Data Gallery
Product Information
KE00285 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TK1 ELISA kit is to be used to detect and quantify protein levels of endogenous human TK1. The assay recognizes human TK1. An antibody specific for human TK1 has been pre-coated onto the microwells. The human TK1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human TK1 is added to detect the captured human TK1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TK1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 24.7 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7083 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| serum | 91% | 82%-101% |
| Cell culture supernatant | 109% | 91%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,484.3 | 187.0 | 7.5 |
| 2 | 20 | 530.4 | 21.7 | 4.1 |
| 3 | 20 | 141.5 | 8.2 | 5.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,464.1 | 193.4 | 7.9 |
| 2 | 24 | 538.7 | 42.8 | 8.0 |
| 3 | 24 | 139.4 | 9.5 | 6.8 |
Background Information
Thymidine kinase 1 (TK1) is a cytosolic enzyme involved in pyrimidine metabolism that catalyzes the addition of a gamma-phosphate group to thymidine and in regenerating thymidine for DNA synthesis and DNA damage. Among the four deoxyribonucleoside-specific kinases in mammalian cells, TK1 is the only one with the most restricted substrates specificity. TK1 has been applied as an important biomarker for the diagnosis of various cancers, including breast cancer, esophageal cancer, and lung cancer. TK1 upregulation was indicated as an early event in a study of breast cancer and further studies demonstrated a positive correlation between TK1 and cancer stage.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | thymidine kinase 1, soluble, Thymidine kinase, cytosolic, TK1, TK2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.