Human TLR6 ELISA Kit
Cat no : KE00734
Synonyms
CD286, toll like receptor 6, Toll-like receptor 6
Validation Data Gallery
Product Information
KE00734 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TLR6 ELISA kit is to be used to detect and quantify protein levels of endogenous human TLR6. The assay recognizes human TLR6. An antibody specific for human TLR6 has been pre-coated onto the microwells. The human TLR6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human TLR6 is added to detect the captured human TLR6 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TLR6 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 1.6 pg/mL |
| Range | 3.9-250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10333 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 109% | 105%-114% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 108.0 | 3.8 | 3.5 |
| 2 | 8 | 43.1 | 1.0 | 2.4 |
| 3 | 8 | 26.8 | 1.3 | 5.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 102.2 | 7.8 | 7.7 |
| 2 | 16 | 42.1 | 1.4 | 3.2 |
| 3 | 16 | 26.6 | 1.2 | 4.4 |
Background Information
TLR6, also known as CD286, belongs to the Toll-like receptor (TLR) family, which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR6 can form a heterodimer with TLR2. TLR6 and TLR2 are recruited to the macrophage phagosome, where they recognize peptidoglycan, a Gram-positive pathogen component.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD286, toll like receptor 6, Toll-like receptor 6 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.