Human TMEM16A/DOG1 ELISA Kit
Cat no : KE00757
Synonyms
ANO1, Anoctamin 1, Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1, DOG1
Validation Data Gallery
Product Information
KE00757 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TMEM16A/DOG1 ELISA kit is to be used to detect and quantify protein levels of endogenous human TMEM16A/DOG1. The assay recognizes human TMEM16A/DOG1. An antibody specific for human TMEM16A/DOG1 has been pre-coated onto the microwells. The human TMEM16A/DOG1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human TMEM16A/DOG1 is added to detect the captured human TMEM16A/DOG1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TMEM16A/DOG1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.4 pg/mL |
| Range | 3.9-250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 55107 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 107% | 82%-122% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 113.8 | 4.4 | 3.9 |
| 2 | 8 | 31.0 | 2.2 | 7.1 |
| 3 | 8 | 17.2 | 0.8 | 4.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 117.5 | 7.0 | 5.9 |
| 2 | 16 | 30.6 | 2.0 | 6.6 |
| 3 | 16 | 16.5 | 1.0 | 6.0 |
Background Information
TMEM16A/DOG1 gene encodes a member of Ca2+ activated Cl- channels (CaCCs) that are critical for physiological functions, such as epithelial secretion, smooth muscle contraction and sensory signal transduction. TMEM16A/DOG1 has been reported to be a valuable target in noncancerous diseases. In cancer, TMEM16A/DOG1 was originally recognized to be positively expressed in gastrointestinal stromal tumor (GIST) and was more well‐recognized as DOG1 (Discovered On GISTs protein 1).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ANO1, Anoctamin 1, Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1, DOG1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.