Human TNC/Tenascin-C ELISA Kit
Cat no : KE00410
Synonyms
Tenascin,TNC,Glioma-associated-extracellular matri
Validation Data Gallery
Product Information
KE00410 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TNC/Tenascin-C ELISA kit is to be used to detect and quantify protein levels of endogenous human TNC/Tenascin-C. The assay recognizes human TNC/Tenascin-C. An antibody specific for human TNC/Tenascin-C has been pre-coated onto the microwells. The human TNC/Tenascin-C protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human TNC/Tenascin-C is added to detect the captured human TNC/Tenascin-C protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TNC/Tenascin-C ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 1.25-80 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3371 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 89% | 73%-113% |
| Cell lysate | 100% | 95%-110% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 40.52 | 2.15 | 5.31 |
| 2 | 20 | 8.74 | 0.33 | 3.74 |
| 3 | 20 | 2.24 | 0.12 | 5.50 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 41.01 | 2.66 | 6.48 |
| 2 | 24 | 8.76 | 0.25 | 2.84 |
| 3 | 24 | 2.28 | 0.16 | 7.13 |
Background Information
Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein of the tenascin family. It is a multimodular protein containing multiple epidermal growth factor (EGF)-like repeats and fibronectin type III (FN III) domains. Tenascin-C is highly expressed during embryonic development, particularly in the developing central nervous system, around motile cells and at epithelial-mesenchymal interaction sites. In adult tissues, the expression and the distribution of TNC are typically limited under normal physiological conditions. It is upregulated during injury, inflammation, regeneration, and cancer. Tenascin-C is a diverse protein that can produce different functions including regulating cell adhesion, migration and proliferation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Tenascin,TNC,Glioma-associated-extracellular matri |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.