Speedy™ Human/Cynomolgus Monkey TNF beta One-Step ELISA Kit
Cat no : SE50234
Synonyms
LTA, LT alpha, Lymphotoxin alpha, Lymphotoxin-alpha, TNF β
Validation Data Gallery
Product Information
| Product name | Speedy™ Human/Cynomolgus Monkey TNF beta One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.1 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human/Cynomolgus Monkey |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4049 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human cell culture supernatant | 104% | 103%-106% |
| Cynomolgus monkey cell culture supernatant | 98% | 94%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 245.0 | 2.5 | 1.0 |
| 2 | 8 | 76.0 | 1.3 | 1.7 |
| 3 | 8 | 43.6 | 1.0 | 2.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 246.5 | 3.6 | 1.5 |
| 2 | 16 | 75.3 | 1.5 | 2.0 |
| 3 | 16 | 42.7 | 1.4 | 3.3 |
Background Information
TNF beta also known as lymphotoxin alpha (LTA), is a member of the tumor necrosis factor family, and is a cytokine produced by lymphocytes. TNF beta is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. TNF beta also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | LTA, LT alpha, Lymphotoxin alpha, Lymphotoxin-alpha, TNF β |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.