Human TNFR1 (Soluble) ELISA Kit
Cat no : KE00309
Synonyms
CD120a, TNFR1, TNFRSF1A, FPF, p55
Validation Data Gallery
Product Information
KE00309 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TNFR1 (Soluble) ELISA kit is to be used to detect and quantify protein levels of endogenous TNFR1 (Soluble). The assay recognizes human TNFR1 (Soluble). An antibody specific for TNFR1 (Soluble) has been pre-coated onto the microwells. The TNFR1 (Soluble) protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-TNFR1 (Soluble) protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TNFR1 (Soluble) ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant，Urine |
| Assay type | Sandwich |
| Sensitivity | 0.4 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7132 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 94% | 77%-128% |
| Cell culture supernatants | 102% | 91%-113% |
| Urine | 99% | 84%-108% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 214.4 | 8.0 | 3.8 |
| 2 | 20 | 52.6 | 2.0 | 3.9 |
| 3 | 20 | 11.9 | 0.8 | 6.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 217.2 | 11.1 | 5.1 |
| 2 | 24 | 51.6 | 1.7 | 3.3 |
| 3 | 24 | 11.4 | 3.9 | 4.9 |
Background Information
Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling. This receptor can be released to the extracellular space by two mechanisms, ectodomain shedding and constitutive release of full-length 55-kDa TNFR1. Soluble TNFR1 (sTNFR1) could function as TNF-binding protein, inhibiting TNF bioactivity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD120a, TNFR1, TNFRSF1A, FPF, p55 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.