Human TNFRSF13B/CD267 ELISA Kit
Cat no : KE00739
Synonyms
CD267, TNFRSF13B, CVID, TACI
Validation Data Gallery
Product Information
KE00739 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TNFRSF13B/CD267 ELISA kit is to be used to detect and quantify protein levels of endogenous human TNFRSF13B/CD267. The assay recognizes human TNFRSF13B/CD267. An antibody specific for human TNFRSF13B/CD267 has been pre-coated onto the microwells. The human TNFRSF13B/CD267 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human TNFRSF13B/CD267 is added to detect the captured human TNFRSF13B/CD267 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TNFRSF13B/CD267 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 23495 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 79% | 75%-82% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 304.4 | 10.8 | 3.6 |
| 2 | 8 | 74.6 | 1.8 | 2.4 |
| 3 | 8 | 32.2 | 1.8 | 5.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 296.5 | 14.6 | 4.9 |
| 2 | 16 | 73.0 | 4.9 | 6.7 |
| 3 | 16 | 31.6 | 2.4 | 7.5 |
Background Information
Tumor necrosis factor receptor superfamily member 13B (TNFRSF13B, also known as CD267) is a member of the tumor necrosis factor receptor superfamily. It is a receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS, binding both ligands with similar high affinity. TNFRSF13B plays a crucial role in humoral immunity by mediating activation of transcription factors and is involved in the stimulation of B- and T-cell function. It also promotes T-cell independent antibody responses and plasma cell differentiation, counteracting BAFF-driven B-cell activation. Additionally, the concentration of plasma TNFSF13B may be correlated with immune thrombocytopenia.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD267, TNFRSF13B, CVID, TACI |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.