Human GITR/TNFRSF18 ELISA Kit
Cat no : KE00220
Synonyms
AITR, CD357, GITR, GITR D, TNFRSF18
Validation Data Gallery
Product Information
KE00220 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TNFRSF18 ELISA kit is to be used to detect and quantify protein levels of endogenous TNFRSF18. The assay recognizes human TNFRSF18. An antibody specific for TNFRSF18 has been pre-coated onto the microwells. The TNFRSF18 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for TNFRSF18 is added to detect the captured TNFRSF18 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GITR/TNFRSF18 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 11.5 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8784 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 83% | 74%-97% |
| Human plasma | 94% | 75%-112% |
| Cell culture supernatants | 110% | 95%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 165.7 | 2.2 | 1.3 |
| 2 | 20 | 663.9 | 13.3 | 2.0 |
| 3 | 20 | 2,812.0 | 99.6 | 3.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 169.7 | 7.2 | 4.3 |
| 2 | 24 | 694.1 | 19.8 | 2.8 |
| 3 | 24 | 2,957.6 | 107.8 | 3.6 |
Background Information
Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells. It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation. GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AITR, CD357, GITR, GITR D, TNFRSF18 |