Speedy™ Human TNFRSF9/CD137 One-Step ELISA Kit
Cat no : SE50085
Synonyms
TNFRSF9, 4 1BB, 4 1BB ligand receptor, 4-1BB ligand receptor, CD137
Validation Data Gallery
Product Information
| Product name | Speedy™ Human TNFRSF9/CD137 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.7 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3604 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 103% | 99%-105% |
| Cell culture supernatant | 91% | 89%-94% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 175.4 | 4.8 | 2.7 |
| 2 | 8 | 44.5 | 0.8 | 1.8 |
| 3 | 8 | 22.7 | 0.2 | 0.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 173.2 | 4.4 | 2.5 |
| 2 | 16 | 44.6 | 0.9 | 2.0 |
| 3 | 16 | 22.6 | 0.3 | 1.3 |
Background Information
CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor which belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. Soluble forms of CD137 (sCD137) are generated by differential splicing and are released by activated lymphocytes. Elevated serum sCD137 level has been associated with some diseases, including rheumatoid arthritis, multiple sclerosis, chronic lymphocytic leukemia, and acute coronary syndrome.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TNFRSF9, 4 1BB, 4 1BB ligand receptor, 4-1BB ligand receptor, CD137 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.