Human TNFRSF9/CD137 ELISA Kit
Cat no : KE00125
Synonyms
TNFRSF9,4 1BB,4 1BB ligand receptor,4-1BB ligand r
Validation Data Gallery
Product Information
KE00125 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TNFRSF9/CD137 ELISA kit is to be used to detect and quantify protein levels of endogenous TNFRSF9/CD137. The assay recognizes human TNFRSF9/CD137. An antibody specific for TNFRSF9/CD137 has been pre-coated onto the microwells. The TNFRSF9/CD137 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for TNFRSF9/CD137 is added to detect the captured TNFRSF9/CD137 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TNFRSF9/CD137 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.0 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3604 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 97% | 77%-122% |
| Cell culture supernatants | 97% | 89%-106% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 241.1 | 7.1 | 2.9 |
| 2 | 20 | 60.3 | 1.1 | 1.8 |
| 3 | 20 | 13.9 | 0.8 | 5.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 246.6 | 14.5 | 5.9 |
| 2 | 24 | 59.2 | 2.4 | 4.1 |
| 3 | 24 | 14.4 | 0.7 | 4.9 |
Background Information
CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor which belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. Soluble forms of CD137 (sCD137) are generated by differential splicing and are released by activated lymphocytes. Elevated serum sCD137 level has been associated with some diseases, including rheumatoid arthritis, multiple sclerosis, chronic lymphocytic leukemia, and acute coronary syndrome.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TNFRSF9,4 1BB,4 1BB ligand receptor,4-1BB ligand r |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.