Human TREM2 ELISA Kit
Cat no : KE00358
Synonyms
TREM 2, TREM-2, Trem2a, Trem2b, Trem2c
Validation Data Gallery
Product Information
KE00358 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TREM2 ELISA kit is to be used to detect and quantify protein levels of endogenous human TREM2. The assay recognizes human TREM2. An antibody specific for human TREM2 has been pre-coated onto the microwells. The human TREM2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human TREM2 is added to detect the captured human TREM2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TREM2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Cell culture supernatants,Cell lysates,Cerebrospinal fluid |
| Assay type | Sandwich |
| Sensitivity | 1.9 pg/mL |
| Range | 23.4-1500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 54209 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 103% | 91%-125% |
| Cell culture supernatants | 88% | 74%-104% |
| Cell lysate | 83% | 74%-96% |
| Human cerebrospinal fluid(CSF) | 90% | 78%-110% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 800.0 | 31.4 | 3.9 |
| 2 | 20 | 179.3 | 8.1 | 4.5 |
| 3 | 20 | 40.4 | 3.8 | 9.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 719.9 | 43.5 | 6.0 |
| 2 | 24 | 167.8 | 7.3 | 4.3 |
| 3 | 24 | 45.4 | 4.1 | 9.1 |
Background Information
TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia. TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles. It associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TREM 2, TREM-2, Trem2a, Trem2b, Trem2c |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.