Human TRIM63 ELISA Kit
Cat no : KE00735
Synonyms
MuRF1, E3 ubiquitin-protein ligase TRIM63, EC:2.3.2.27, IRF, Iris RING finger protein
Validation Data Gallery
Product Information
KE00735 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TRIM63 ELISA kit is to be used to detect and quantify protein levels of endogenous human TRIM63. The assay recognizes human TRIM63. An antibody specific for human TRIM63 has been pre-coated onto the microwells. The human TRIM63 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human TRIM63 is added to detect the captured human TRIM63 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TRIM63 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 84676 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 88% | 77%-96% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2.51 | 0.13 | 5.27 |
| 2 | 8 | 0.55 | 0.02 | 4.42 |
| 3 | 8 | 0.28 | 0.02 | 5.92 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2.57 | 0.21 | 8.31 |
| 2 | 16 | 0.56 | 0.03 | 4.53 |
| 3 | 16 | 0.29 | 0.01 | 4.82 |
Background Information
TRIM63, also named as IRF, MURF1, RNF28 and SMRZ, is E3 ubiquitin ligase. It is regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. TRIM63 may play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. It may regulate the organization of myofibrils through TTN in muscle cells. TRIM63 is in muscle-specific expression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MuRF1, E3 ubiquitin-protein ligase TRIM63, EC:2.3.2.27, IRF, Iris RING finger protein |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.