Human TRP1 ELISA Kit
Cat no : KE00594
Synonyms
TYRP1, 5,6-dihydroxyindole-2-carboxylic acid oxidase, b PROTEIN, CAS2, Catalase B
Validation Data Gallery
Product Information
KE00594 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TRP1 ELISA kit is to be used to detect and quantify protein levels of endogenous human TRP1. The assay recognizes human TRP1. An antibody specific for human TRP1 has been pre-coated onto the microwells. The human TRP1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human TRP1 is added to detect the captured human TRP1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TRP1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7306 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 82% | 80%-84% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 6.15 | 0.39 | 6.39 |
| 2 | 8 | 2.48 | 0.19 | 7.76 |
| 3 | 8 | 2.22 | 0.05 | 2.04 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 8.42 | 0.80 | 9.45 |
| 2 | 16 | 2.11 | 0.17 | 7.93 |
| 3 | 16 | 1.10 | 0.11 | 9.94 |
Background Information
TYRP1 (Tyrosinase-related protein 1) also named as TRP1, is a protein related to tyrosinase and is primarily involved in melanin synthesis. It is expressed in melanocytes and is responsible for catalyzing the conversion of dopaquinone to melanin, making it one of the key enzymes in the melanin synthesis process. Mutations in TYRP1 are associated with various pigment-related diseases, including OCA3 (Oculocutaneous Albinism Type 3). Additionally, TYRP1 plays an important role in animal models; for example, mutations in TYRP1 in the Mexican axolotl (Ambystoma mexicanum) result in the 'copper' phenotype.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TYRP1, 5,6-dihydroxyindole-2-carboxylic acid oxidase, b PROTEIN, CAS2, Catalase B |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.