Human Total MMP-3 Antibody Pair Set
Cat no : PA00150
Synonyms
Validation Data Gallery
Product Information
| Product name | Human Total MMP-3 Antibody Pair Set |
| Tests | 10 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 3.5h |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4314 |
Recovery
| Sample Type | Average | Range |
|---|
IntraAssay
| Sample | n | mean () | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? | ? | ? |
| 2 | 20 | ? | ? | ? |
| 3 | 20 | ? | ? | ? |
InterAssay
| Sample | n | mean () | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | ? | ? | ? |
| 2 | 24 | ? | ? | ? |
| 3 | 24 | ? | ? | ? |
Background Information
MMP-3 (matrix metallopeptidase 3), also named as Stromelysin 1 or STR1, is a member of matrix metalloproteinase (MMP) family. The MMP family of enzymes is comprised of critically important extracellular matrix remodeling proteases whose activity has been implicated in normal embryogenesis and tissue remodelling and in many diseases such as arthritis, cancer, periodontitis, glomerulonephritis, encephalomyelitis, atherosclerosis and tissue ulceration. These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 is secreted from the cells as a proenzyme. The proenzyme has been shown to stimulate plasminogen activation. The active MMP-3 is capable of cleaving types III, IV, IX and X collagen, aggrecan, fbronectin, laminin, IGFBP-3, serpins, and IL-1β.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.