Human UTX ELISA Kit
Cat no : KE00693
Synonyms
[histone H3]-trimethyl-L-lysine(27) demethylase 6A, EC:1.14.11.68, Histone demethylase UTX, KDM6A, Lysine specific demethylase 6A
Validation Data Gallery
Product Information
KE00693 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human UTX ELISA kit is to be used to detect and quantify protein levels of endogenous human UTX. The assay recognizes human UTX. An antibody specific for human UTX has been pre-coated onto the microwells. The human UTX protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human UTX is added to detect the captured human UTX protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human UTX ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7403 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 114% | 101%-126% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 10.91 | 0.53 | 4.86 |
| 2 | 8 | 2.29 | 0.12 | 5.04 |
| 3 | 8 | 1.16 | 0.03 | 2.91 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 10.70 | 0.51 | 4.76 |
| 2 | 16 | 2.27 | 0.09 | 4.18 |
| 3 | 16 | 1.15 | 0.06 | 5.12 |
Background Information
UTX protein, also known as KDM6A, is a histone demethylase whose main function is to remove the methylation modification of lysine 27 (H3K27) on histone H3, thereby activating the transcription of target genes and playing a vital role in epigenetic regulation. UTX plays an important role in embryonic development and cell differentiation, affecting cell fate determination and tissue-specific function by regulating the expression of specific genes. This protein is a component of the COMPASS-like complex, which includes the histone methyltransferase MLL4 (KMT2D) and other related proteins.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | [histone H3]-trimethyl-L-lysine(27) demethylase 6A, EC:1.14.11.68, Histone demethylase UTX, KDM6A, Lysine specific demethylase 6A |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.