Human VEGF-D ELISA Kit
Cat no : KE00247
Synonyms
c Fos induced growth factor, FIGF, Vascular endothelial growth factor-D, VEGF D, VEGFD, VEGF-D
Validation Data Gallery
Product Information
KE00247 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The VEGF-D ELISA kit is to be used to detect and quantify protein levels of endogenous VEGF-D. The assay recognizes human VEGF-D. An antibody specific for VEGF-D has been pre-coated onto the microwells. The VEGF-D protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human VEGF-D is added to detect the captured human VEGF-D protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human VEGF-D ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 6.7 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2277 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 100% | 76%-113% |
| Cell culture supernatants | 112% | 87%-128% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,028.8 | 63.9 | 6.2 |
| 2 | 20 | 240.2 | 11.3 | 4.7 |
| 3 | 20 | 56.5 | 3.5 | 6.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,060.9 | 45.8 | 4.3 |
| 2 | 24 | 263.7 | 13.5 | 5.1 |
| 3 | 24 | 61.6 | 3.2 | 5.1 |
Background Information
Vascular endothelial growth factor-D (VEGF-D) is a secreted glycoprotein that can activate VEGF receptors on the endothelium, is a mitogen for endothelial cells and promotes the growth and remodeling of blood vessels and lymphatic vessels. VEGF-D undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. VEGF-D has been established to be over-expressed in both tumor tissues and patients' serum samples in several types of human cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | c Fos induced growth factor, FIGF, Vascular endothelial growth factor-D, VEGF D, VEGFD, VEGF-D |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.