Human WAPL ELISA Kit
Cat no : KE00838
Synonyms
FOE, WAPL/FOE, KIAA0261, WAPAL, WAPL cohesin release factor
Validation Data Gallery
Product Information
KE00838 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human WAPL ELISA kit is to be used to detect and quantify protein levels of endogenous human WAPL. The assay recognizes human WAPL. An antibody specific for human WAPL has been pre-coated onto the microwells. The human WAPL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human WAPL is added to detect the captured human WAPL protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human WAPL ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.47-30 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 23063 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 101% | 73%-120% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 18.92 | 0.51 | 2.71 |
| 2 | 8 | 4.25 | 0.32 | 7.54 |
| 3 | 8 | 2.51 | 0.07 | 2.82 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 18.53 | 1.22 | 6.59 |
| 2 | 16 | 4.21 | 0.37 | 8.80 |
| 3 | 16 | 2.23 | 0.31 | 14.14 |
Background Information
WAPL protein regulates binding of the cohesin complex to chromosomes during interphase and helps remove cohesin from chromosomes at mitosis. By dissociating cohesin from chromatin WAPL is a key regulator in a cycle of loading and unloading that is collectively referred to as cohesin turnover. WAPL is required for various cellular process including sister chromatid resolution and DNA repair.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FOE, WAPL/FOE, KIAA0261, WAPAL, WAPL cohesin release factor |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.