Human WISP-1/CCN4 ELISA Kit
Cat no : KE00236
Synonyms
CCN family member 4, CCN4, WISP 1, WISP1, WISP1c, WISP1i, WISP1tc, Wnt 1 induced secreted protein
Validation Data Gallery
Product Information
KE00236 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The WISP-1 ELISA kit is to be used to detect and quantify protein levels of endogenous WISP-1. The assay recognizes human WISP-1. An antibody specific for WISP-1 has been pre-coated onto the microwells. The WISP-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human WISP-1 is added to detect the captured human WISP-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human WISP-1/CCN4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.4 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8840 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 87% | 73%-99% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 459.3 | 15.1 | 3.3 |
| 2 | 20 | 116.0 | 3.3 | 2.8 |
| 3 | 20 | 25.6 | 1.4 | 5.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 466.8 | 20.0 | 4.3 |
| 2 | 24 | 117.2 | 4.3 | 3.6 |
| 3 | 24 | 25.5 | 1.7 | 6.7 |
Background Information
CCN4, also known as WISP-1, is a member of the WNT1 inducible signaling pathway protein (WISP) subfamily of the connective tissue growth factor/CCN family of matricellular proteins. CCN4 is both an intracellular and a secreted protein found in the extracellular matrix and is present throughout the body. Like many other matricellular proteins, CCN4 can also regulate cellular responses such as cell growth, differentiation and survival. Additionally, CCN4 plays a multiple physiologic roles in development and also participates in pathogenesis. CCN4 is associated with cancer and can serve as a biomarker or prognostic factor as well as a potential therapeutic target.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CCN family member 4, CCN4, WISP 1, WISP1, WISP1c, WISP1i, WISP1tc, Wnt 1 induced secreted protein |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Oncogene The multifunctional RNA helicase DDX39A drives glioblastoma progression by modulating WISP1 alternative splicing that induces an immunosuppressive macrophage polarization. |