Human ZAG/AZGP1 ELISA Kit
Cat no : KE00230
Synonyms
AZGP1, ZA2G, ZAG, Zinc alpha 2 glycoprotein, Zn alpha 2 glycoprotein, Zn alpha 2 GP, ZNGP1
Validation Data Gallery
Product Information
KE00230 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The AZGP1 ELISA kit is to be used to detect and quantify protein levels of endogenous AZGP1. The assay recognizes human AZGP1. An antibody specific for AZGP1 has been pre-coated onto the microwells. The AZGP1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human AZGP1 is added to detect the captured human AZGP1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ZAG/AZGP1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 13.6 pg/mL |
| Range | 187.5-6000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 563 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 92% | 79%-125% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,802.7 | 136.2 | 4.9 |
| 2 | 20 | 694.9 | 24.8 | 3.6 |
| 3 | 20 | 322.3 | 19.9 | 6.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,923.0 | 139.1 | 4.8 |
| 2 | 24 | 726.1 | 25.6 | 3.5 |
| 3 | 24 | 357.2 | 17.2 | 4.8 |
Background Information
Zinc-alpha-2-glycoprotein (AZGP1) is a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor. It is synthesized by adipocytes and epithelial cells of many organs, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens. AZGP1 plays a role in lipid metabolism, and is also involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AZGP1, ZA2G, ZAG, Zinc alpha 2 glycoprotein, Zn alpha 2 glycoprotein, Zn alpha 2 GP, ZNGP1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Signal Transduct Target Ther Enhancing KLF15 activity in cardiomyocytes: a novel approach to prevent pathological reprogramming and fibrosis via nuclease-deficient dCas9VPR. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kristian (Verified Customer) (12-04-2024) | Works great, and is specific for human ZAG over mouse ZAG.
 |