Human ZNF148 ELISA Kit
Cat no : KE00773
Synonyms
BERF 1, BFCOL1, HT BETA, pHZ 52, Transcription factor ZBP 89
Validation Data Gallery
Product Information
KE00773 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ZNF148 ELISA kit is to be used to detect and quantify protein levels of endogenous human ZNF148. The assay recognizes human ZNF148. An antibody specific for human ZNF148 has been pre-coated onto the microwells. The human ZNF148 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ZNF148 is added to detect the captured human ZNF148 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ZNF148 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 1.1 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7707 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 99% | 92%-115% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 924.2 | 33.8 | 3.7 |
| 2 | 8 | 243.7 | 8.1 | 3.3 |
| 3 | 8 | 122.7 | 3.8 | 3.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 936.4 | 38.0 | 4.1 |
| 2 | 16 | 242.4 | 9.4 | 3.9 |
| 3 | 16 | 119.1 | 5.5 | 4.7 |
Background Information
The Kruppel-like zinc finger protein 148 (ZNF148), also known as β enolase repressor factor 1 (BERF1, ZBP-89, and BFCOL1), encoded by this gene is a member of the Kruppel family of zinc finger DNA binding proteins. It plays critical roles in gene regulation by binding to GC-rich DNA sequences, influencing cellular processes such as proliferation, differentiation, apoptosis, and stress responses. ZNF148 is expressed at lower levels in mammalian somatic cells, and highly expressed in tumor cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BERF 1, BFCOL1, HT BETA, pHZ 52, Transcription factor ZBP 89 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.