Human ZNF217 ELISA Kit
Cat no : KE00928
Synonyms
ZFP217, ZABC1, Zinc finger protein 217
Validation Data Gallery
Product Information
KE00928 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ZNF217 ELISA kit is to be used to detect and quantify protein levels of endogenous human ZNF217. The assay recognizes human ZNF217. An antibody specific for human ZNF217 has been pre-coated onto the microwells. The human ZNF217 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ZNF217 is added to detect the captured human ZNF217 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ZNF217 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.34 ng/mL |
| Range | 0.39-25 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7764 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 88% | 86%-90% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 13.38 | 1.10 | 8.25 |
| 2 | 8 | 2.96 | 0.22 | 7.35 |
| 3 | 8 | 1.40 | 0.12 | 8.35 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 12.65 | 1.13 | 8.91 |
| 2 | 16 | 2.96 | 0.16 | 5.52 |
| 3 | 16 | 1.43 | 0.13 | 9.19 |
Background Information
Zinc finger protein 217 (ZNF217) is a member of the Krüppel-like factor transcription factor family. ZNF217 possesses a characteristic structure of zinc finger motifs and plays a crucial role in regulating the biological activities of cells. ZNF217 binds to DNA through its zinc fingers, thereby targeting potential protein binding partners to specific sites in the genome. Recent findings have revealed that ZNF217 is strongly associated with multiple aspects of cancer progression, impacting patient prognosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ZFP217, ZABC1, Zinc finger protein 217 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.