Human PGⅡ ELISA Kit
Cat no : KE00090
Synonyms
Gastricsin, Pepsinogen C, PEPSINOGEN, GROUP II, PGC, Progastricsin, progastricsin (pepsinogen C)
Validation Data Gallery
Product Information
KE00090 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PGⅡ ELISA kit is to be used to detect and quantify protein levels of endogenous PGⅡ. The assay recognizes human PGⅡ. An antibody specific for PGⅡ has been pre-coated onto the microwells. The PGⅡ protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for PGⅡ is added to detect the captured PGⅡprotein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PGⅡ ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 1.56-100 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5225 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 99% | 75%-128% |
| Cell culture supernatants | 92% | 86%-101% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 5.4 | 0.2 | 4.3 |
| 2 | 20 | 25.2 | 0.9 | 3.4 |
| 3 | 20 | 89.9 | 6.0 | 6.7 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 5.6 | 0.2 | 3.1 |
| 2 | 24 | 24.4 | 0.6 | 2.4 |
| 3 | 24 | 80.8 | 4.9 | 6.1 |
Background Information
PepsinogenⅠand Ⅱ, as precursors of pepsin, are produced by gastric mucosa and released into the gastric lumen and peripheral circulation. The advanced inflammation of gastric mucosa and its progression toward atrophic gastritis (AG) in the corpus is associated with a change in serum biomarkers pepsinogenⅠand Ⅱ.(PMID:23496362). PepsinogenⅡ also known as progastricsin or PGC (pepsinogen C), is an aspartic protease expressed primarily in gastric chief cells and is a novel marker of type 2 cells with advantages over many of the current markers used to identify type 2 cells in the developing lung(PMID:14578117). It is involved in proteolysis and peptidolysis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Gastricsin, Pepsinogen C, PEPSINOGEN, GROUP II, PGC, Progastricsin, progastricsin (pepsinogen C) |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.