Human t-Plasminogen activator ELISA Kit
Sensitivity
8.9 pg/mL
Range
156.3 - 10000 pg/mL, 78.1 - 5000 pg/mL
Reactivity
Human
Cat no : KE00180
Synonyms
Alteplase, DKFZp686I03148, plasminogen activator, tissue, PLAT, Reteplase, T PA, t plasminogen activator, TPA
Validation Data Gallery
Product Information
KE00180 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The t-Plasminogen activator ELISA kit is to be used to detect and quantify protein levels of endogenous t-Plasminogen activator. The assay recognizes human t-Plasminogen activator. An antibody specific for t-Plasminogen activator has been pre-coated onto the microwells. The t-Plasminogen activator protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for t-Plasminogen activator is added to detect the captured t-Plasminogen activator protein. For signal development, horseradish peroxidase (HRP)-conjugated is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human t-Plasminogen activator ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 8.9 pg/mL |
| Range | 156.3 - 10000 pg/mL, 78.1 - 5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5327 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 84% | 74%-93% |
| Plasma | 92% | 78%-125% |
| cell culture supernatants | 87% | 76%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,076.2 | 110.5 | 5.3 |
| 2 | 20 | 797.3 | 56.7 | 7.1 |
| 3 | 20 | 538.5 | 49.2 | 9.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,214.8 | 168.9 | 7.6 |
| 2 | 24 | 814.1 | 71.9 | 8.8 |
| 3 | 24 | 539.8 | 34.2 | 6.3 |
Background Information
Plasminogen activator, tissue (PLAT, synonyms: TPA, T-PA) is a tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein (33 kDa and 32 kDa). PLAT enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. tPA has 4 isoforms produced by alternative splicing with the MW of 63 kDa, 33 kDa, 57 kDa and 44 kDa.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Alteplase, DKFZp686I03148, plasminogen activator, tissue, PLAT, Reteplase, T PA, t plasminogen activator, TPA |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Molecules Bioevaluation of Pheretima vulgaris Antithrombotic Extract, PvQ, and Isolation, Identification of Six Novel PvQ-Derived Fibrinolytic Proteases. | ||
J Control Release Vascular endothelial cell-targeted mRNA delivery via synthetic lipid nanoparticles for venous thrombosis prevention |