IAH1 Polyclonal antibody

IAH1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17808-1-AP

Synonyms

IAH1

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Validation Data Gallery

• 

  WB analysis of mouse testis using 17808-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17808-1-AP (IAH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17808-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17808-1-AP (IAH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17808-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17808-1-AP (IAH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 17808-1-AP

  IP result of anti-IAH1 (IP:17808-1-AP, 3ug; Detection:17808-1-AP 1:500) with mouse kidney tissue lysate 5000ug.

• 

  IP experiment of mouse testis using 17808-1-AP

  IP result of anti-IAH1 (IP:17808-1-AP, 3ug; Detection:17808-1-AP 1:500) with mouse testis tissue lysate 4800ug.

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse brain tissue, mouse kidney tissue
• Positive IP detected in: mouse kidney tissue, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17808-1-AP targets IAH1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IAH1 fusion protein Ag12089
• Full Name: isoamyl acetate-hydrolyzing esterase 1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 248 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC111025
• Gene Symbol: IAH1
• Gene ID (NCBI): 285148
• RRID: AB_2123524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Proteomics; Increased expression of peroxiredoxin 1 and identification of a novel lipid-metabolizing enzyme in the early phase of liver ischemia reperfusion injury.; Authors - Wilson Claire H CH