IARS Polyclonal antibody

IARS Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26942-1-AP

Synonyms

IARS, IARS1, IleRS, ILRS, IRS, Isoleucine tRNA ligase, isoleucyl tRNA synthetase, PRO0785



Tested Applications

Positive WB detected inK-562 cells, U2OS cells
Positive IP detected inU2OS cells
Positive IHC detected inhuman testis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26942-1-AP is IARS Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IARS fusion protein Ag25686
Full Name isoleucyl-tRNA synthetase
Calculated Molecular Weight 144 kDa
Observed Molecular Weight 145 kDa
GenBank Accession NumberBC065552
Gene Symbol IARS
Gene ID (NCBI) 3376
RRIDAB_2880693
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for IARS antibody 26942-1-APDownload protocol
IHC protocol for IARS antibody 26942-1-APDownload protocol
IP protocol for IARS antibody 26942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Food Chem Toxicol

The crucial role of thioredoxin interacting protein in the liver insulin resistance induced by di (2-ethylhexyl) phthalates.

Authors - Wang Zhang
mouseWB

Sci Rep

Intermittent fasting activates macrophage migration inhibitory factor and alleviates high-fat diet-induced nonalcoholic fatty liver disease

Authors - Dezhao Li
humanWB,IF

Discov Oncol

High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer

Authors - Haiqing Wang
humanIF

Nat Commun

Time-resolved proteomic profiling reveals compositional and functional transitions across the stress granule life cycle

Authors - Shuyao Hu
humanWB

Proc Natl Acad Sci U S A

Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex

Authors - Krishnendu Khan
  • KD Validated