Tested Applications
| Positive WB detected in | mouse lung tissue, mouse spleen tissue |
| Positive IP detected in | mouse spleen tissue |
| Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse lung tissue |
| Positive IF/ICC detected in | 4T1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16174-1-AP targets ICAM-1/CD54 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | intercellular adhesion molecule 1 |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 90-110 kDa |
| GenBank Accession Number | NM_010493 |
| Gene Symbol | ICAM-1 |
| Gene ID (NCBI) | 15894 |
| RRID | AB_2248702 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13597 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Where is ICAM-1 expressed?
Intercellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54) is a transmembrane glycoprotein constitutively expressed at low levels in endothelial cells, pericytes and on some lymphocytes and monocytes1. It is located at the cytoplasmic membrane, with a large extracellular region of mainly hydrophobic amino acids joined to a small transmembrane region and a cytoplasmic tail. It has a molecular weight of 75 to 115 kDa depending on the level of glycosylation.
What is the function of ICAM-1?
ICAM-1 is important in both innate and adaptive immune responses as an adhesion molecule. Although it is constitutively expressed, in the presence of pro-inflammatory cytokines such as TNFα the endothelial cells are activated and upregulate expression of ICAM-12. In blood vessels lined with endothelial cells, leukocytes that are rolling over the surface are able to bind to ICAM-1 and transmigrate through the endothelial barrier and into the tissue. The initial binding of the leukocytes to ICAM-1 causes a Ca2+ release that initiates endothelial cell contraction and weakening of the intercellular tight junctions3, 4. This protein can be used as an indicator of endothelial activation and of vascular inflammation.
What is the role of ICAM-1 in disease?
Beyond the role in the immune response, ICAM-1 has also been identified as the target of attachment for the human rhinovirus, the cause of the common cold. Binding of the virus to ICAM-1 causes the viral capsid to uncoat and leads to release of the genetic material5.
Hubbard, A. K. & Rothlein, R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radic. Biol. Med. 28, 1379-86 (2000).
Long, E. O. ICAM-1: getting a grip on leukocyte adhesion. J. Immunol. 186, 5021-3 (2011).
Lawson, C. & Wolf, S. ICAM-1 signaling in endothelial cells. (2009).
Lyck, R. & Enzmann, G. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues. Curr. Opin. Hematol. 22, 53-59 (2015).
Xing, L., Casasnovas, J. M. & Cheng, R. H. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating. J. Virol. 77, 6101-7 (2003).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ICAM-1/CD54 antibody 16174-1-AP | Download protocol |
| IHC protocol for ICAM-1/CD54 antibody 16174-1-AP | Download protocol |
| IP protocol for ICAM-1/CD54 antibody 16174-1-AP | Download protocol |
| WB protocol for ICAM-1/CD54 antibody 16174-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ICAM-1/CD54 Polyclonal antibody (Cat# 16174-1-AP) has accumulated 48 citations across journals including Nature Communications, Science Advances, Cell Reports, Advanced Science, Journal of Controlled Release, Cell Death & Disease, Pharmacological Research, Atherosclerosis, Journal of Neuroinflammation, and Stem Cell Research & Therapy. Research fields span cardiovascular disease (atherosclerosis, myocardial ischemia-reperfusion), neuroinflammation and blood-brain barrier biology, diabetic complications (nephropathy, retinopathy, cardiomyopathy), sepsis, cancer, wound healing, neuropathic pain, asthma, pre-eclampsia, and endothelial cell biology, with primary applications in Western blot, immunofluorescence, and immunohistochemistry.
| Species | Application | Title |
|---|---|---|
Nat Commun NNMT/1-MNA protects against hepatic ischemia-reperfusion injury through the AKT/FOXO1/ANGPT2/JNK axis | ||
J Adv Res Engineering a multifunctional nanoplatform with cascade catalytic and bidirectional immunomodulatory capacities for integrating sepsis therapy. | ||
J Nanobiotechnology Early assessment and treatment of ventricular remodeling in vivo via a targeted ultrasonic molecular probe loaded with oxygen and cholecystokinin | ||
Adv Sci (Weinh) Astrocyte-Derived Extracellular Vesicular miR-143-3p Dampens Autophagic Degradation of Endothelial Adhesion Molecules and Promotes Neutrophil Transendothelial Migration after Acute Brain Injury | ||
Sci Adv Vagus nerve stimulation modulates distinct acetylcholine receptors on B cells and limits the germinal center response. | ||
J Control Release Multifunctional nanoparticles for immune regulation and oxidative stress alleviation in myocarditis |
