ICAM-1/CD54 Recombinant monoclonal antibody

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  WB analysis using 82827-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82827-1-RR (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 82827-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82827-1-RR (ICAM-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 82827-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82827-1-RR (ICAM-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 82827-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82827-1-RR (ICAM-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 82827-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82827-1-RR (ICAM-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ICAM-1/CD54 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 82827-1-RR

Clone No.2E23

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CD54, ICAM-1, MALA-2, Icam1, ICAM 1

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse spleen tissue, rat lung tissue
• Positive IHC detected in: mouse lung tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

82827-1-RR targets ICAM-1/CD54 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 85-110 kDa
• GenBank Accession Number: NM_010493
• Gene Symbol: ICAM-1
• Gene ID (NCBI): 15894
• RRID: AB_3670566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13597
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Microbiol Res; Trojan horse strategy and TfR/ LDLR-Mediated transcytosis determine the dissemination of mycobacteria in tuberculous meningoencephalitis; Authors - Ziyi Liu