ICAM-1/CD54 Recombinant monoclonal antibody

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  WB analysis using 83069-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83069-6-RR (ICAM-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 83069-6-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ICAM-1 antibody (83069-6-RR, Clone: 230342A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 83069-6-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ICAM-1 antibody (83069-6-RR, Clone: 230342A2 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 83069-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83069-6-RR against Human ICAM-1 were performed. The affinity constant is 0.271 nM.

ICAM-1/CD54 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83069-6-RR

Clone No.230342A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

CD54, ICAM-1, ICAM1, ICAM 1, BB2

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Tested Applications

• Positive WB detected in: HUVEC cells, Raji cells, Daudi cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83069-6-RR targets ICAM-1/CD54 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 85-95 kDa
• GenBank Accession Number: BC015969
• Gene Symbol: ICAM-1
• Gene ID (NCBI): 3383
• ENSEMBL Gene ID: ENSG00000090339
• RRID: AB_3670791
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05362
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).