ICAM-1/CD54 Recombinant antibody

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  WB analysis using 83696-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83696-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat liver using 83696-3-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 83696-3-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat lung using 83696-3-RR

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 83696-3-RR

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat liver using 83696-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ICAM-1/CD54 antibody (83696-3-RR, Clone: 240725C2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83696-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83696-3-RR against Mouse ICAM-1/CD54 were performed. The affinity constant is 1.09 nM.

ICAM-1/CD54 {ptg:Brand} Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 83696-3-RR

Clone No.240725C2

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

CD54, ICAM-1, ICAM-12, Icam1, 240725C2

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Tested Applications

• Positive WB detected in: rat lung tissue, rat liver tissue, rat heart tissue, rat kidney tissue, rat spleen tissue
• Positive IHC detected in: rat liver tissue, rat lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83696-3-RR targets ICAM-1/CD54 in WB, IHC, IF-P, ELISA applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: NM_012967.1
• Gene Symbol: Icam1
• Gene ID (NCBI): 25464
• RRID: AB_3671299
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q00238
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).