ICAM-2/CD102 Recombinant monoclonal antibody

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  WB analysis using 84442-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84442-1-RR (ICAM-2/CD102 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 84442-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84442-1-RR (ICAM-2/CD102 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 84442-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84442-1-RR (ICAM-2/CD102 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 84442-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84442-1-RR (ICAM-2/CD102 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84442-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84442-1-RR against Mouse ICAM-2/CD102 were performed. The affinity constant is below 1 pM.

ICAM-2/CD102 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 84442-1-RR

Clone No.241387F11

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

ICAM-2, ICAM2, ICAM 2, CD102, 241387F11

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Tested Applications

• Positive WB detected in: mouse lung tissue, bEnd.3 cells
• Positive IHC detected in: mouse cerebellum tissue, mouse lung tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84442-1-RR targets ICAM-2/CD102 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: intercellular adhesion molecule 2
• Calculated Molecular Weight: 31kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: NM_010494.2
• Gene Symbol: Icam2
• Gene ID (NCBI): 15896
• RRID: AB_3671967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P35330
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187.