ICAM-2/CD102 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 84857-3-RR (same clone as 84857-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84857-3-RR (ICAM-2/CD102 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84857-3-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 84857-3-RR (same clone as 84857-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using ICAM-2/CD102 antibody (84857-3-RR, Clone: 242237G11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84857-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01646-2

  Cytometric bead array standard curve of MP01646-2, ICAM2/CD102 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84857-3-PBS. Detection antibody: 84857-4-PBS. Standard: Eg2002. Range: 0.625-80 ng/mL.

ICAM-2/CD102 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 84857-3-PBS

Clone No.242237G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

CD102, ICAM2, 242237G11, ICAM 2, ICAM-2

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Product Information

84857-3-PBS targets ICAM-2/CD102 as part of a matched antibody pair:

MP01646-2: 84857-3-PBS capture and 84857-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ICAM-2/CD102 fusion protein Eg2002
• Full Name: intercellular adhesion molecule 2
• Calculated Molecular Weight: 31kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_001099789.2
• Gene Symbol: ICAM2/CD102
• Gene ID (NCBI): 3384
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13598
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187.