Tested Applications
| Positive WB detected in | mouse kidney tissue, mouse lung tissue, mouse brain tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82827-8-RR targets ICAM-1/CD54 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | intercellular adhesion molecule 1 |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 85-110 kDa |
| GenBank Accession Number | NM_010493 |
| Gene Symbol | ICAM-1 |
| Gene ID (NCBI) | 15894 |
| RRID | AB_3670567 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P13597 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ICAM-1/CD54 antibody 82827-8-RR | Download protocol |
| IHC protocol for ICAM-1/CD54 antibody 82827-8-RR | Download protocol |
| WB protocol for ICAM-1/CD54 antibody 82827-8-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ICAM-1/CD54 Recombinant monoclonal antibody (240249H6), catalog number 82827-8-RR, has 5 total citations. These appear in J Nanobiotechnology, Theranostics, Adv Healthc Mater, J Transl Med, and Genes Dis. Research fields span retinal neovascularization, acute liver injury, antimicrobial nanoparticle therapy for bacterial infections, angiogenesis and programmed cell death in spinal cord injury, and atherosclerosis via exosomal miRNA-mediated endothelial inflammation.
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology Charge-flip nanoparticles loaded with TAK1 inhibitors inhibit retinal neovascularization. | ||
Theranostics Nanotuner targeting mitochondrial redox and iron homeostasis imbalance for the treatment of acute liver injury | ||
Adv Healthc Mater De Novo Development of Bioresponsive Nanoparticles With Antimicrobial Activity to Treat Bacterial Infections. | ||
J Transl Med Integration of angiogenesis and programmed cell death mechanisms unveils potential diagnostic and therapeutic targets in spinal cord injury. | ||
Genes Dis Targeting hepatic CREBZF protects against atherosclerosis via exosomal miR-210-3p mediated inhibition of endothelial inflammation. |
