ICAM2/CD102 Polyclonal antibody

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  WB analysis using 27322-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27322-1-AP (ICAM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 27322-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 27322-1-AP (ICAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 27322-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27322-1-AP (ICAM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ICAM2/CD102 Polyclonal Antibody for WB, ELISA

Cat No. 27322-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

ICAM2, CD102, ICAM-2, ICAM 2

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Tested Applications

• Positive WB detected in: HL-60 cells, mouse lung tissue, Jurkat cells, HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27322-1-AP targets ICAM2/CD102 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25952

  Product name: Recombinant human ICAM2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 65-223 aa of BC003097

  Sequence: DKILLDEQAQWKHYLVSNISHDTVLQCHFTCSGKQESMNSNVSVYQPPRQVILTLQPTLVAVGKSFTIECRVPTVEPLDSLTLFLFRGNETLHYETFGKAAPAPQEATATFNSTADREDGHRNFSCLAVLDLMSRGGNIFHKHSAPKMLEIYEPVSDSQ

• Full Name: intercellular adhesion molecule 2
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 55-80 kDa
• GenBank Accession Number: BC003097
• Gene Symbol: ICAM2/CD102
• Gene ID (NCBI): 3384
• RRID: AB_2880843
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13598
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187. This antibody got 55-80 kDa in western blotting maybe due to glycosylation.