ICAM2 Polyclonal antibody

ICAM2 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC,ELISA

Conjugate

Unconjugated

Cat no : 27322-1-AP

Synonyms

CD102, ICAM 2, ICAM2



Tested Applications

Positive WB detected inHL-60 cells, mouse lung tissue, Jurkat cells, HUVEC cells
Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27322-1-AP is ICAM2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ICAM2 fusion protein Ag25952
Full Name intercellular adhesion molecule 2
Calculated molecular weight 31 kDa
Observed molecular weight 55-80 kDa
GenBank accession numberBC003097
Gene symbol ICAM2
Gene ID (NCBI) 3384
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187. This antibody got 55-80 kDa in western blotting maybe due to glycosylation.

Protocols

Product Specific Protocols
WB protocol for ICAM2 antibody 27322-1-APDownload protocol
FC protocol for ICAM2 antibody 27322-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols