ICAM3 Polyclonal antibody

ICAM3 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 17699-1-AP

Synonyms

CD50, CDW50, ICAM 3, ICAM R, ICAM3



Tested Applications

Positive WB detected inJurkat cells, Ramos cells, U-937 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17699-1-AP targets ICAM3 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ICAM3 fusion protein Ag11955
Full Name intercellular adhesion molecule 3
Calculated molecular weight 547 aa, 60 kDa
Observed molecular weight 110-160 kDa, 60 kDa
GenBank accession numberBC058903
Gene symbol ICAM3
Gene ID (NCBI) 3385
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ICAM3 antibody 17699-1-APDownload protocol
IHC protocol for ICAM3 antibody 17699-1-APDownload protocol
IP protocol for ICAM3 antibody 17699-1-APDownload protocol
FC protocol for ICAM3 antibody 17699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols