ICAM3 Polyclonal antibody

ICAM3 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 17699-1-AP

Synonyms

CD50, CDW50, ICAM 3, ICAM R, ICAM3



Tested Applications

Positive WB detected inJurkat cells, Ramos cells, U-937 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17699-1-AP targets ICAM3 in WB, IP, IHC, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ICAM3 fusion protein Ag11955
Full Name intercellular adhesion molecule 3
Calculated Molecular Weight 547 aa, 60 kDa
Observed Molecular Weight 110-160 kDa, 60 kDa
GenBank Accession NumberBC058903
Gene Symbol ICAM3
Gene ID (NCBI) 3385
RRIDAB_2248763
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ICAM3 antibody 17699-1-APDownload protocol
IHC protocol for ICAM3 antibody 17699-1-APDownload protocol
IP protocol for ICAM3 antibody 17699-1-APDownload protocol
FC protocol for ICAM3 antibody 17699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols