ICAM3/CD50 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 84335-5-RR (same clone as 84335-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84335-5-RR (ICAM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84335-5-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 84335-5-RR (same clone as 84335-5-PBS)

  WB result of ICAM3/CD50 antibody (84335-5-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ICAM3/CD50 transfected Jurkat cells. This data was developed using the same antibody clone with 84335-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84335-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84335-5-RR against Human ICAM3/CD50 were performed. The affinity constant is 24.7 nM.

• Featured Product
• KD/KO Validated

ICAM3/CD50 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 84335-5-PBS

Clone No.241417F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

CDW50, ICAM3, 241417F3, CD50, ICAM 3

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Product Information

84335-5-PBS targets ICAM3/CD50 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  fusion protein

• Full Name: intercellular adhesion molecule 3
• Calculated Molecular Weight: 547 aa, 60 kDa
• Observed Molecular Weight: 110-130 kDa
• GenBank Accession Number: BC058903
• Gene Symbol: ICAM3/CD50
• Gene ID (NCBI): 3385
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P32942
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Intercellular Adhesion Molecule 3 (ICAM-3), also known as CD50, is a member of the immunoglobulin superfamily. It is a transmembrane glycoprotein that contains an N-terminus extracellular domain, a single transmembrane domain, and a C-terminus cytoplasmic domain. ICAM-3 possesses 2-9 Ig-like C2-type domains in its extracellular region and is known to bind to the leukocyte adhesion protein LFA-1 . This molecule is expressed on leukocytes and epidermal Langerhans cells and plays a significant role in immune cell interactions and T lymphocyte activation.