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CoraLite® Plus 488-conjugated ICAM4 Monoclonal antibody

ICAM4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3A9B9

Cat no : CL488-67014

Synonyms

CD242, ICAM 4, ICAM4, LW, LW blood group protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human spleen tissue using CoraLite® Plus 488-conjugated ICAM4 Monoclonal ant (CL488-67014)

    IF Staining of human spleen using CL488-67014

    Immunofluorescent analysis of (4% PFA) fixed human spleen tissue using CoraLite® Plus 488 ICAM4 antibody (CL488-67014, Clone: 3A9B9 ) at dilution of 1:1000.

Tested Applications

Positive IF detected inhuman spleen tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67014 targets ICAM4 in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ICAM4 fusion protein Ag27628
Full Name intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)
Calculated Molecular Weight 271 aa, 29 kDa
GenBank Accession NumberBC029364
Gene Symbol ICAM4
Gene ID (NCBI) 3386
RRIDAB_2919405
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes the Landsteiner-Wiener (LW) blood group antigen(s) that belongs to the immunoglobulin (Ig) superfamily, and that shares similarity with the intercellular adhesion molecule (ICAM) protein family. The protein migrate as a 42 kDa glycoprotein on SDS PAGE. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ICAM4 antibody CL488-67014Download protocol
Standard Protocols
Click here to view our Standard Protocols