CoraLite®594-conjugated ICAM4 Monoclonal antibody

ICAM4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3A9B9

Cat no : CL594-67014

Synonyms

CD242, ICAM 4, ICAM4, LW, LW blood group protein

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Validation Data Gallery

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  IF Staining of mouse spleen using CL594-67014

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite®594 ICAM4 antibody (CL594-67014, Clone: 3A9B9 ) at dilution of 1:200.

• 

  IF Staining of mouse spleen using CL594-67014

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite®594 ICAM4 antibody (CL594-67014, Clone: 3A9B9 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse spleen tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67014 targets ICAM4 in IF applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ICAM4 fusion protein Ag27628
• Full Name: intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)
• Calculated Molecular Weight: 271 aa, 29 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC029364
• Gene Symbol: ICAM4
• Gene ID (NCBI): 3386
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes the Landsteiner-Wiener (LW) blood group antigen(s) that belongs to the immunoglobulin (Ig) superfamily, and that shares similarity with the intercellular adhesion molecule (ICAM) protein family. The protein migrate as a 42 kDa glycoprotein on SDS PAGE. Alternative splicing results in multiple transcript variants encoding distinct isoforms.